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KMID : 0613820070170050719
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Journal of Life Science
2007 Volume.17 No. 5 p.719 ~ p.722
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Construction of the Transgenic Drosophila melanogaster Expressing a Human Megsin Gene
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Goo Tae-Won
Kwon Ki-Sang
Kwon O-Yu
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Abstract
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IgA nephropathy (IgAN) is considered to be a multifactorial disease with genetic and environmental factors contributing to its pathogenesis. The genes involved in susceptibility and progression of the disease have not yet been clearly elucidated. Megsin is an important candidate gene, predominantly expressed in glomerular mesangium and upregulated in IgAN. To understand biological function of megsin, in this work we have produced transgenic D. melanogaster fly over-expressing human megsin (actin-ga14＞UAS-Megsin fly). Introduced human megsin was confirmed by RT-PCR and Western blotting, respectively. Its phenotype is melanin deficiency-abdomen and the megsin gene is stably transferred to the next generations.
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KEYWORD
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IgA nephropathy(IgAN), Drosophila melanogaster, megsin
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